Recombinant RPS19 Antibody (Ab125911) - Western Blot All lanes: Recombinant RPS19 Antibody (Ab125911) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2.0 s
Recombinant RPS19 Antibody (Ab125911) - IF/ICC IF analysis of Recombinant RPS19 Antibody (Ab125911) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant RPS19 Antibody (Ab125911) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab138205) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).