Recombinant RPS3 Antibody (Ab125945) - Western Blot All lanes: Recombinant RPS3 Antibody (Ab125945) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 27 kDa Observed band size: 33 kDa Exposure time: 49.5 s
Recombinant RPS3 Antibody (Ab125945) - Western Blot All lanes: Recombinant RPS3 Antibody (Ab125945) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 27 kDa Observed band size: 33 kDa Exposure time: 23.1 s
Recombinant RPS3 Antibody (Ab125945) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant RPS3 Antibody (Ab125945). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant RPS3 Antibody (Ab125945) - IF/ICC IF analysis of Recombinant RPS3 Antibody (Ab125945) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant RPS3 Antibody (Ab125945) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).