Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell .Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
宿主种属
兔(Rabbit)
特异性
RPS9
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse),牛(Cow),猪(Pig)
免疫原
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RPS9.
阳性对照
WB: Jurkat, HEK293, HeLa, NIH/3T3 and C6 cell lysates. IHC: Human brain tissue. IF/ICC: Jurkat cells.
RPS9 Antibody (Ab125986) - Western Blot All lanes: RPS9 Antibody (Ab125986) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 22 kDa Observed band size: 25 kDa Exposure time: 100.0 s
RPS9 Antibody (Ab125986) - IHC Immunohistochemical analysis of RPS9 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the RPS9 Antibody (Ab125986) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
RPS9 Antibody (Ab125986) - IF/ICC Immunofluorescent analysis of RPS9 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X - 100 in TBS for 5 - 10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with RPS9 Antibody (Ab125986) in 3% BSA - PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594 -conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).