Rabbit anti Human RSK2 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500 - 1/1000 IHC: 1/50-1/100 Protein Function Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. Post-translational Autophosphorylated on Ser-386, as part of the activation process. Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.
规格
产品名称
Recombinant RSK2 Antibody
别名
重组 RSK2 抗体
英文别名
90 kDa ribosomal protein S6 kinase 3 | EC 2.7.11.1 | Insulin-stimulated protein kinase 1 | ISPK-1 | MAP kinase-activated protein kinase 1b | MAPK-activated protein kinase 1b | MAPKAP kinase 1b | MAPKAPK-1b | p90-RSK 3 | p90RSK3 | pp90RSK2 | Ribosomal S6 k
规格或纯度
ExactAb™, Validated, 重组, 0.1 mg/mL
宿主种属
兔(Rabbit)
特异性
RPS6KA3
种属反应性
人(Human),仓鼠(Hamster),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide of human Rsk 2/MAPKAP Kinase 1b.
阳性对照
WB: Ramos, Hep G2, HeLa, A549, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue. IF/ICC: HeLa cells.
Recombinant RSK2 Antibody (Ab126046) - Western Blot All lanes: Recombinant RSK2 Antibody (Ab126046) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 84 kDa Observed band size: 89 kDa Exposure time: 2.0 s
Recombinant RSK2 Antibody (Ab126046) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant RSK2 Antibody (Ab126046). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant RSK2 Antibody (Ab126046) - IF/ICC
IF analysis of Recombinant RSK2 Antibody (Ab126046) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant RSK2 Antibody (Ab126046) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
ZJ23F1202157
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