Rabbit anti Human RSK1 p90 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
规格
产品名称
Recombinant RSK1 p90 Antibody
别名
丝氨酸/苏氨酸激酶p90RSK蛋白单克隆抗体 | 重组 RSK1 p90 抗体
英文别名
90kD, polypeptide 1) | EC 2.7.11 | EC 2.7.11.1,90 kDa ribosomal protein S6 kinase 1 | HU-1 | MAP kinase-activated protein kinase 1a | MAPK-activated protein kinase 1a | MAPKAP kinase 1a | MAPKAPK-1a | MAPKAPL1A | p90RSK1 | p90S6K | ribosomal protein S6 ki
规格或纯度
ExactAb™, Validated, 重组, 1.5 mg/mL
宿主种属
兔(Rabbit)
特异性
RPS6KA1
种属反应性
人(Human)
免疫原
A synthetic peptide derived from human RSK1 p90 (AA 1-20)
阳性对照
WB: A431, MCF7 and K562 cell lysates. IHC: Human Cholangiocarcinoma tissue. IF/ICC: HeLa cells.
Recombinant RSK1 p90 Antibody (Ab126061) - Western Blot All lanes: Recombinant RSK1 p90 Antibody (Ab126061) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 83 kDa Observed band size: 82 kDa Exposure time: 31.1 s
Recombinant RSK1 p90 Antibody (Ab126061) - IHC Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant RSK1 p90 Antibody (Ab126061). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant RSK1 p90 Antibody (Ab126061) - IF/ICC
IF analysis of Recombinant RSK1 p90 Antibody (Ab126061) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant RSK1 p90 Antibody (Ab126061) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).