Rabbit anti Human RYK Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.
Recombinant RYK Antibody (Ab126166) - Western Blot All lanes: Recombinant RYK Antibody (Ab126166) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 68 kDa Observed band size: 67, 68, 77 kDa Exposure time: 8.7 s
Recombinant RYK Antibody (Ab126166) - IF/ICC Immunocytochemistry analysis of RYK (green) in Hela using Recombinant RYK Antibody (Ab126166) and DAPI (blue).
Recombinant RYK Antibody (Ab126166) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant RYK Antibody (Ab126166). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.