Rabbit anti Human SAE1 Antibody, Recombinant, could be used for WB, IF, ICC and so on. Application: WB: 1/500-1/1000 IF/ICC: 1/50-1/200 Protein Function The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
规格
产品名称
Recombinant SAE1 Antibody
别名
泛素样蛋白SUMO1激活酶抗体 | 重组SAE1抗体
英文别名
Activator of SUMO1 | AOS 1 | AOS1 | FLJ3091 | HSPC 140 | HSPC140 | SAE 1 | Sae1 | SAE1_HUMAN | Sentrin/SUMO activating protein AOS 1 | Sentrin/SUMO activating protein AOS1 | SUA 1 | SUA1 | SUMO 1 activating enzyme E1 N subunit | SUMO 1 activating enzyme s
规格或纯度
ExactAb™, Validated, 重组, 1.5 mg/mL
宿主种属
兔(Rabbit)
特异性
SAE1
种属反应性
人(Human),小鼠(Mouse)
免疫原
A synthetic peptide derived from human SAE1 (AA 65-78)
阳性对照
WB: HeLa, Jurkat, HEK293, MCF7 and NIH/3T3 cell lysates. IF/ICC: HeLa cells.
Recombinant SAE1 Antibody (Ab126360) - Western Blot All lanes: Recombinant SAE1 Antibody (Ab126360) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 65.2 s
Recombinant SAE1 Antibody (Ab126360) - IF/ICC Immunocytochemistry analysis of SAE1 (green) in HeLa using Recombinant SAE1 Antibody (Ab126360), and DAPI (blue).