The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
宿主种属
兔(Rabbit)
特异性
UBA2
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human UBA2 (AA 50-100)
阳性对照
WB: A549, K562, HeLa, RAW 264.7 and C6 cell lysates.
Recombinant SAE2/UBA2 Antibody (Ab126366) - Western Blot All lanes: Recombinant SAE2/UBA2 Antibody (Ab126366) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 71 kDa Observed band size: 84 kDa Exposure time: 38.6 s
Recombinant SAE2/UBA2 Antibody (Ab126366) - Western Blot All lanes: Recombinant SAE2/UBA2 Antibody (Ab126366) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 71 kDa Observed band size: 84 kDa Exposure time: 38.6 s