Rabbit anti Human SAFB Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 IF/ICC: 1/50-1/200 Protein Function Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Can inhibit cell proliferation.
规格
产品名称
Recombinant SAFB Antibody
别名
SAFB蛋白抗体 | 重组SAFB抗体
英文别名
SAFB | HAP | HET | SAFB1 | Scaffold attachment factor B1 | SAF-B | SAF-B1 | HSP27 estrogen response element-TATA box-binding protein | HSP27 ERE-TATA-binding protein | DKFZp779C1727 antibody | glutathione S transferase fusion protein antibody | HAP antibo
规格或纯度
ExactAb™, Validated, 重组, 0.5 mg/mL
宿主种属
兔(Rabbit)
特异性
SAFB
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human SAFB (AA 902-915)
阳性对照
WB: Mouse brain, K562, and C6 lysates. IHC: Human breast cancer tissue. IF/ICC: 293 cells.
Recombinant SAFB Antibody (Ab126368) - Western Blot All lanes: Recombinant SAFB Antibody (Ab126368) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 103 kDa Observed band size: 150 kDa Exposure time: 10.5 s
Recombinant SAFB Antibody (Ab126368) - Western Blot All lanes: Recombinant SAFB Antibody (Ab126368) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 103 kDa Observed band size: 149 kDa Exposure time: 92.8 s
Recombinant SAFB Antibody (Ab126368) - IF/ICC Immunocytochemistry analysis of SAFB1 (green) in 293 using Recombinant SAFB Antibody (Ab126368) and DAPI (blue).
Recombinant SAFB Antibody (Ab126368) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Recombinant SAFB Antibody (Ab126368). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.