ExactAb™,已验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
| Rabbit anti Human SC35 Antibody, Recombinant, could be used for WB, IHC and so on. |
| Application: |
| WB: 1/500-1/1000 IHC: 1/50-1/100 IF/ICC: 1/200 |
| Protein Function: |
| Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. |
Recombinant SC35 Antibody (Ab126662) - Western Blot
All lanes: Recombinant SC35 Antibody (Ab126662) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 25 kDa
Observed band size: 32 kDa
Exposure time: 8.0 s
Recombinant SC35 Antibody (Ab126662) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Recombinant SC35 Antibody (Ab126662). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant SC35 Antibody (Ab126662) - IF/ICC
IF analysis of Recombinant SC35 Antibody (Ab126662) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant SC35 Antibody (Ab126662) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
1/200
1/500-1/1000
1/50-1/100
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0910073 |
分析证书 | Ab126662 |
| ZJ24F0910074 |
分析证书 | Ab126662 |
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