Rabbit anti Human SCRN1 Antibody, Polyclonal, could be used for WB, IHC and so on. Application WB: 1/500-1/2000 IHC: 1/50-1/300
IF/ICC: 1/500 Protein Function Regulates exocytosis in mast cells. Increases both the extent of secretion and the sensitivity of mast cells to stimulation with calcium.
SCRN1 Antibody (Ab126819) - Western Blot All lanes: SCRN1 Antibody (Ab126819) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 46 kDa Observed band size: 41 kDa Exposure time: 19.7 s
SCRN1 Antibody (Ab126819) - IHC Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. SCRN1 Antibody (Ab126819) was diluted at 1/200 (4℃ overnight). Tris-EDTA, pH 9.0 was used for antigen retrieval. Secondary antibody was diluted at 1/200 (room temperature, 45 min).
SCRN1 Antibody (Ab126819) - IF/ICC IF analysis of SCRN1 Antibody (Ab126819) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with SCRN1 Antibody (Ab126819) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab138205) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).