Rabbit anti Human SDHB Antibody, Recombinant, could be used for WB, IP and so on.
规格
产品名称
Recombinant SDHB Antibody
别名
SDHB抗体 | 铬细胞瘤患者抑癌基因抗体 | 琥珀酸脱氢酶复合体B亚基抗体
英文别名
CWS2 antibody | DHSB_HUMAN antibody | FLJ92337 antibody | Ip antibody | Iron sulfur subunit antibody | Iron sulfur subunit of complex II antibody | Iron-sulfur subunit of complex II antibody | mitochondrial antibody | PGL 4 antibody | PGL4 antibody | SDH
规格或纯度
重组, ExactAb™, 已验证, 见COA
生化机理
Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
宿主种属
兔(Rabbit)
特异性
SDHB
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human SDHB (AA 1-280).
阳性对照
WB: Mouse slpeen, Rat spleen, HeLa, Jurkat and C6 lysates. IP: HeLa lysates. IF/ICC: HeLa cells.
Recombinant SDHB Antibody (Ab126854) - Western Blot All lanes: Recombinant SDHB Antibody (Ab126854) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 32 kDa Observed band size: 28, 24 kDa The band observed at 24 kDa could potentially be a cleaved form of SDHB due to the presence of a 28 amino acid transit peptide. Exposure time: 12.4 s
Recombinant SDHB Antibody (Ab126854) - IP Immunoprecipitate (IP) analysis using Recombinant SDHB Antibody (Ab126854) at 1/50 dilution. (WB with Recombinant SDHB Antibody (Ab126854) at 1/1000 dilution).
Recombinant SDHB Antibody (Ab126854) - IF/ICC IF analysis of Recombinant SDHB Antibody (Ab126854) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant SDHB Antibody (Ab126854) at 1/300 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
ZJ24F1013212
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