ExactAb™,已验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human SET/TAF-I Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/50-1/200
Protein Function:
Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
Recombinant SET/TAF-I Antibody (Ab127229) - Western Blot
All lanes: Recombinant SET/TAF-I Antibody (Ab127229) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 38, 34 kDa
Recombinant SET/TAF-I Antibody (Ab127229) recognizes different isoforms of human SET/TAF-I.
Exposure time: 4.9 s
Recombinant SET/TAF-I Antibody (Ab127229) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant SET/TAF-I Antibody (Ab127229). Highpressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant SET/TAF-I Antibody (Ab127229) - IF/ICC
Immunocytochemistry analysis of SET(green) in HeLa using Recombinant SET/TAF-I Antibody (Ab127229), and DAPI(blue).
1/50 - 1/100
1/50 - 1/200
1/500-1/1000
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
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通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ25F0218577 |
分析证书 | Ab127229 |
| ZJ25F0218576 |
分析证书 | Ab127229 |
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