ExactAb™,已验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human SF2 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function:
Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors.
Recombinant SF2 Antibody (Ab127257) - Western Blot
All lanes: Recombinant SF2 Antibody (Ab127257) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 30 kDa
Exposure time: 2.2 s
Recombinant SF2 Antibody (Ab127257) - IF/ICC
Immunocytochemistry analysis of SF2 (green) in 293T using Recombinant SF2 Antibody (Ab127257) and DAPI (blue).
Recombinant SF2 Antibody (Ab127257) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant SF2 Antibody (Ab127257). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
1/50 - 1/100
1/50-1/200
1/500-1/1000
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0809806 |
分析证书 | Ab127257 |
| ZJ24F0809807 |
分析证书 | Ab127257 |
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