ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human SH3KBP1 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/200
Protein Function
Adapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through a association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit (By similarity). May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis.
SH3KBP1 Antibody (Ab127404) - Western Blot
All lanes: SH3KBP1 Antibody (Ab127404) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 73 kDa
Observed band size: 80 kDa
Exposure time: 1.0 s
SH3KBP1 Antibody (Ab127404) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using SH3KBP1 Antibody (Ab127404). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
SH3KBP1 Antibody (Ab127404) - IF/ICC
IF analysis of SH3KBP1 Antibody (Ab127404) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with SH3KBP1 Antibody (Ab127404) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
1/200
1/500-1/1000
1/50-1/100
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0911005 |
分析证书 | Ab127404 |
| ZJ24F0911006 |
分析证书 | Ab127404 |
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