Rabbit anti Human SHMT2 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on. Application: WB: 0.01-2µg/mL IHC: 5-20µg/mL ICC/IF: 5-20µg/mL Protein Function Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA.
规格
产品名称
SHMT2/SHMT Antibody
别名
丝氨酸羟甲基转移酶2(SHMT2)多克隆抗体 | SHMT2/SHMT 抗体
英文别名
EC 2.1.2.1 | Glycine hydroxymethyltransferase | Serine methylase | SHMT | Epididymis secretory sperm binding protein Li 51e antibody | GLY A+ antibody | GLYA antibody | glycine auxotroph A, human complement forhamster antibody | Glycine hydroxymethyltrans
规格或纯度
无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属
兔(Rabbit)
特异性
SHMT2
种属反应性
人(Human),猪(Pig)
免疫原
Recombinant SHMT2 expressed in E.coli (AA 30-504)
阳性对照
WB: MCF7, MDA-MB-231, HeLa and HEK293 cell lysates. IHC: Human breast cancer, stomach, stomach cancer, colorectal cancer, prostate, cerebrum tissues. ICC/IF: HeLa, MCF7 cells.
偶联
Unconjugated
克隆类型
多克隆抗体
Format
Whole IgG
亚型
Rabbit IgG
纯化方法
Antigen affinity purified + Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4
防腐剂
0.05% Proclin 300
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
图片
SHMT2/SHMT Antibody (Ab127481) - Western Blot All lanes: SHMT2/SHMT Antibody (Ab127481) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 55 kDa Observed band size: 53, 56 kDa Exposure time: 3.8 s
SHMT2/SHMT Antibody (Ab127481) - Western Blot All lanes: SHMT2/SHMT Antibody (Ab127481) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 55 kDa Observed band size: 53 kDa Exposure time: 3.8 s
SHMT2/SHMT Antibody (Ab127481) - IF/ICC FITC staining on IF Sample: HeLa cells. Primary Ab: 20 µg/L SHMT2/SHMT Antibody (Ab127481) Second Ab: 2 μg/mL AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
SHMT2/SHMT Antibody (Ab127481) - IF/ICC AF488 staining on IF Sample: MCF7 cell Primary Ab: 20 µg/L SHMT2/SHMT Antibody (Ab127481) Second Ab: 2 μg/mL AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
SHMT2/SHMT Antibody (Ab127481) - IHC DAB staining on IHC Samples: Human Breast cancer Tissue Primary Ab: 20 µg/mL SHMT2/SHMT Antibody (Ab127481) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
SHMT2/SHMT Antibody (Ab127481) - IHC DAB staining on IHC Samples: Human Stomach Tissue Primary Ab: 20 µg/mL SHMT2/SHMT Antibody (Ab127481) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
SHMT2/SHMT Antibody (Ab127481) - IHC DAB staining on IHC Samples: Human Stomach cancer Tissue Primary Ab: 20 µg/mL SHMT2/SHMT Antibody (Ab127481) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
SHMT2/SHMT Antibody (Ab127481) - IHC DAB staining on IHC Samples: Human Colorectal cancer Tissue Primary Ab: 20 µg/mL SHMT2/SHMT Antibody (Ab127481) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
SHMT2/SHMT Antibody (Ab127481) - IHC DAB staining on IHC Samples: Human Prostate Tissue Primary Ab: 20 µg/mL SHMT2/SHMT Antibody (Ab127481) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
SHMT2/SHMT Antibody (Ab127481) - IHC DAB staining on IHC Samples: Human Cerebrum Tissue Primary Ab: 20 µg/mL SHMT2/SHMT Antibody (Ab127481) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.