Rabbit anti Human SHP1 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function: Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
Recombinant SHP1 Antibody (Ab127501) - Western Blot All lanes: Recombinant SHP1 Antibody (Ab127501) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 67 kDa Observed band size: 56, 65 kDa Exposure time: 17.1 s
Recombinant SHP1 Antibody (Ab127501) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant SHP1 Antibody (Ab127501). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant SHP1 Antibody (Ab127501) - IF/ICC
IF analysis of Recombinant SHP1 Antibody (Ab127501) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant SHP1 Antibody (Ab127501) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
ZJ24F0102376
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