Rabbit anti Human SIRP alpha Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Signal regulatory protein alpha type 1 antibody | Bit antibody | Brain Ig like molecule with tyrosine based activation motifs antibody | Brain Ig-like molecule with tyrosine-based activation motifs antibody | Brain immunoglobulin like molecule with tyrosi
规格或纯度
ExactAb™, Validated, 重组, 0.7 mg/mL
宿主种属
兔(Rabbit)
特异性
SIRPA
种属反应性
人(Human)
免疫原
Recombinant protein of human SIRP alpha (AA 31-373)
阳性对照
WB: HEK293, A549, U937 and THP-1 cell lysates. ICC/IF: HeLa cells.
Recombinant SIRP alpha Antibody (Ab127634) - Western Blot All lanes: Recombinant SIRP alpha Antibody (Ab127634) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 55 kDa Observed band size: 74 kDa Exposure time: 65.4 s
Recombinant SIRP alpha Antibody (Ab127634) - Western Blot All lanes: Recombinant SIRP alpha Antibody (Ab127634) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 55 kDa Observed band size: 77 kDa Exposure time: 30.0 s
Recombinant SIRP alpha Antibody (Ab127634) - IF Immunocytochemistry analysis of SIRP alpha (green) in HeLa using Recombinant SIRP alpha Antibody (Ab127634), and DAPI (blue).