ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human Smoothened Antibody, Polyclonal, could be used for WB, IHC and so on.
Smoothened Antibody (Ab128394) - Western Blot
All lanes: Smoothened Antibody (Ab128394) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 86 kDa
Observed band size: 75, 89 kDa
The expression profile and molecular weight observed may detect the protein form without the signal peptide.
Exposure time: 68.4 s
Smoothened Antibody (Ab128394) - IHC
Immunohistochemical analysis of Smoothened staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Smoothened Antibody (Ab128394) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Smoothened Antibody (Ab128394) - IF/ICC
IF analysis of Smoothened Antibody (Ab128394) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Smoothened Antibody (Ab128394) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
1/500-1/1000
1/100 - 1/200
1/200
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1114227 |
分析证书 | Ab128394 |
| ZJ24F1114228 |
分析证书 | Ab128394 |
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