Rabbit anti Human SMYD3 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.
规格
产品名称
Recombinant SMYD3 Antibody
别名
组蛋白甲基转移酶SMYD3抗体 | 重组SMYD3抗体
英文别名
bA74P14.1 (novel protein) antibody | bA74P14.1 antibody | FLJ21080 antibody | histone lysine N methyltransferase SMYD3 antibody | KMT3E antibody | MGC104324 antibody | SET and MYND domain containing 3 antibody | SET and MYND domain containing protein 3 an
规格或纯度
ExactAb™, Validated, 重组, 1.6 mg/mL
宿主种属
兔(Rabbit)
特异性
SMYD3
种属反应性
人(Human),小鼠(Mouse)
免疫原
A synthetic peptide derived from human SMYD3 (AA 270-284)
阳性对照
WB: Jurkat, HeLa, K562 and NIH/3T3 cell lysates. IHC: Human lung cancer tissue. ICC/IF: K562 cells.
Recombinant SMYD3 Antibody (Ab128421) - Western Blot All lanes: Recombinant SMYD3 Antibody (Ab128421) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 49 kDa Observed band size: 44 kDa Exposure time: 22.1 s
Recombinant SMYD3 Antibody (Ab128421) - Western Blot All lanes: Recombinant SMYD3 Antibody (Ab128421) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 49 kDa Observed band size: 44 kDa Exposure time: 40.0 s
Recombinant SMYD3 Antibody (Ab128421) - IF/ICC Immunocytochemistry analysis of SMYD3 (green) in K562 using Recombinant SMYD3 Antibody (Ab128421) and DAPI (blue).
Recombinant SMYD3 Antibody (Ab128421) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant SMYD3 Antibody (Ab128421). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.