ExactAb™,已验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human SPAK Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/200
Protein Function
May act as a mediator of stress-activated signals.
Recombinant SPAK Antibody (Ab128888) - Western Blot
All lanes: Recombinant SPAK Antibody (Ab128888) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 59 kDa
Observed band size: 56, 59, 63 kDa
Exposure time: 49.5 s
Recombinant SPAK Antibody (Ab128888) - Western Blot
All lanes: Recombinant SPAK Antibody (Ab128888) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 59 kDa
Observed band size: 63 kDa
Exposure time: 37.3 s
Recombinant SPAK Antibody (Ab128888) - IHC
Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant SPAK Antibody (Ab128888). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant SPAK Antibody (Ab128888) - IF/ICC
IF analysis of Recombinant SPAK Antibody (Ab128888) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant SPAK Antibody (Ab128888) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
1/500-1/1000
1/50 - 1/100
1/200
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0607286 |
分析证书 | Ab128888 |
| ZJ24F0607287 |
分析证书 | Ab128888 |
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