ExactAb™,已验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human SRP19 Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/200
IF/ICC: 1/200
Protein Function：
Signal-recognition-particle assembly, binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP.
Recombinant SRP19 Antibody (Ab129186) - Western Blot
All lanes: Recombinant SRP19 Antibody (Ab129186) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 16 kDa
Observed band size: 24 kDa
Exposure time: 0.8 s
Recombinant SRP19 Antibody (Ab129186) - IF/ICC
Immunohistochemical analysis of SRP19 staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Recombinant SRP19 Antibody (Ab129186) at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).
Recombinant SRP19 Antibody (Ab129186) - IF/ICC
IF analysis of Recombinant SRP19 Antibody (Ab129186) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant SRP19 Antibody (Ab129186) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
1/50-1/200
1/200
1/500-1/1000
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0910585 |
分析证书 | Ab129186 |
| ZJ24F0910586 |
分析证书 | Ab129186 |
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