Rabbit anti Human StAR Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 0.01-2µg/mL
IHC: 5-20µg/mL
IF/ICC: 0.54µg/mL
Protein Function
Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
规格
产品名称
StAR Antibody
别名
类固醇合成急性调节蛋白(STAR)多克隆抗体 | StAR抗体
英文别名
StAR | StARD1 | START domain-containing protein 1 | Cholesterol trafficker antibody | Luteinizing hormone induced protein antibody | mitochondrial antibody | Mitochondrial steroid acute regulatory protein antibody | StAR antibody | StAR related lipid tran
规格或纯度
无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属
兔(Rabbit)
特异性
STAR
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse),猪(Pig)
免疫原
Recombinant STAR expressed in E.coli (AA 1-285)
阳性对照
WB: K562 and THP-1 cell lysates. IHC: Human kidney tissue. IF/ICC: HeLa cells.
偶联
Unconjugated
克隆类型
多克隆抗体
Format
Whole IgG
亚型
Rabbit IgG
纯化方法
Antigen affinity purified + Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4
防腐剂
0.05% Proclin 300
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
图片
StAR Antibody (Ab129336) - Western Blot All lanes: StAR Antibody (Ab129336) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 32 kDa Observed band size: 28 kDa Exposure time: 20.0 s
StAR Antibody (Ab129336) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 20 μg/mL StAR Antibody (Ab129336) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
StAR Antibody (Ab129336) - IF/ICC
IF analysis of StAR Antibody (Ab129336) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with StAR Antibody (Ab129336) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).