Recombinant HSF1 Antibody (Ab129534) - Western Blot All lanes: Recombinant HSF1 Antibody (Ab129534) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 56 kDa Observed band size: 47 kDa Exposure time: 100.0 s
Recombinant HSF1 Antibody (Ab129534) - IHC Immunohistochemical analysis of STEAP2 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Recombinant HSF1 Antibody (Ab129534) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Recombinant HSF1 Antibody (Ab129534) - IF/ICC Immunofluorescent analysis of STEAP2 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Recombinant HSF1 Antibody (Ab129534) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).