Recombinant Superoxide Dismutase 1 Antibody (Ab129785) - Western Blot All lanes: Recombinant Superoxide Dismutase 1 Antibody (Ab129785) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 16 kDa Observed band size: 17 kDa Exposure time: 3.1 s
Recombinant Superoxide Dismutase 1 Antibody (Ab129785) - IHC Immunohistochemical analysis of Superoxide Dismutase 1staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Recombinant Superoxide Dismutase 1 Antibody (Ab129785) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Recombinant Superoxide Dismutase 1 Antibody (Ab129785) - IF/ICC Immunofluorescent analysis of Superoxide Dismutase 1 staining in HCT116 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Recombinant Superoxide Dismutase 1 Antibody (Ab129785) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.