Rabbit anti Human TCF7L2 Antibody, Recombinant, could be used for WB, IHC and so on.
规格
产品名称
Recombinant TCF7L2 Antibody
别名
E2 2; HMG box transcription factor 4; hTCF 4; Immunoglobulin transcription factor 2; ITF2; SEF2 1; SEF2 1A; SEF2 1B; SEF2; T cell factor 4; T cell specific HMG box; T cell specific transcription factor 4; TCF 4; TCF4; TCF7L2; TCF7L2 protein; hTCF-4; T-cel
规格或纯度
重组, ExactAb™, 已验证, 见COA
生化机理
Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.
宿主种属
兔(Rabbit)
特异性
TCF7L2
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human TCF7L2 (AA 1-50)
阳性对照
WB: Jurkat, K562, HeLa, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human colonic cancer tissue. IF/ICC: HeLa cells.
Recombinant TCF7L2 Antibody (Ab130508) - Western Blot All lanes: Recombinant TCF7L2 Antibody (Ab130508) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 68 kDa Observed band size: 62 kDa Exposure time: 6.5 s
Recombinant TCF7L2 Antibody (Ab130508) - IHC Immunohistochemical analysis of paraffin-embedded human colonic cancer, using Recombinant TCF7L2 Antibody (Ab130508).
Recombinant TCF7L2 Antibody (Ab130508) - IF/ICC IF analysis of Recombinant TCF7L2 Antibody (Ab130508) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant TCF7L2 Antibody (Ab130508) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).