Rabbit anti Human TCTEX-1 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function: Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Binds to transport cargos and is involved in apical cargo transport such as rhodopsin-bearing vesicles in polarized epithelia. Is involved in intracellular targeting of D-type retrovirus gag polyproteins to the cytoplasmic assembly site. May also be a accessory component of axonemal dynein. Plays a role in neuronal morhpogenesis; the function is independent of cytoplasmic dynein and seems to be coupled to regulation of the actin cytoskeleton by enhancing Rac1 activity. The function in neurogenesis may be regulated by association with a G-protein beta-gamma dimer. May function as a receptor-independent activator of heterotrimeric G-protein signaling; the activation appears to be independent of a nucleotide exchange. Plays a role in regulating neurogenesis; inhibits the genesis of neurons from precursor cells during cortical development presumably by antagonizing ARHGEF2. Involved in the regulation of mitotic spindle orientation.
Recombinant TCTEX-1 Antibody (Ab130575) - Western Blot All lanes: Recombinant TCTEX-1 Antibody (Ab130575) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 12 kDa Observed band size: 14 kDa Exposure time: 2.8 s
Recombinant TCTEX-1 Antibody (Ab130575) - IF/ICC Immunocytochemistry analysis of TCTEX-1 (green) in HeLa using Recombinant TCTEX-1 Antibody (Ab130575) and DAPI (blue).
Recombinant TCTEX-1 Antibody (Ab130575) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant TCTEX-1 Antibody (Ab130575). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.