Recombinant TEMT Antibody (Ab130682) - Western Blot All lanes: Recombinant TEMT Antibody (Ab130682) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 28 kDa Observed band size: 27 kDa Exposure time: 12.4 s
Recombinant TEMT Antibody (Ab130682) - Western Blot All lanes: Recombinant TEMT Antibody (Ab130682) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 28 kDa Observed band size: 27 kDa Exposure time: 16.9 s
Recombinant TEMT Antibody (Ab130682) - IF/ICC Immunofluorescent analysis of INMT staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Recombinant TEMT Antibody (Ab130682) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).