Rabbit anti Human TFII I Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.
规格
产品名称
Recombinant TFII I Antibody
别名
蛋白酪氨酸激酶BAP135抗体 | 重组TFII I抗体
英文别名
BAP 135 antibody | BAP-135 antibody | BAP135 antibody | Bruton tyrosine kinase associated protein 135 antibody | Bruton tyrosine kinase-associated protein 135 antibody | BTK associated protein 135 antibody | BTK associated protein 135kD antibody | BTK ass
规格或纯度
ExactAb™, Validated, 重组, 0.2 mg/mL
宿主种属
兔(Rabbit)
特异性
GTF2I
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human TFII I (AA 66-80)
阳性对照
WB: HEEK293, A549, HeLa, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue. ICC/IF: HeLa cells.
Recombinant TFII I Antibody (Ab130795) - Western Blot All lanes: Recombinant TFII I Antibody (Ab130795) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 112 kDa Observed band size: 129 kDa Exposure time: 14.7 s
Recombinant TFII I Antibody (Ab130795) - IF/ICC Immunocytochemistry analysis of General Transcription Factor II I (green) in HeLa using Recombinant TFII I Antibody (Ab130795) and DAPI (blue).
Recombinant TFII I Antibody (Ab130795) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant TFII I Antibody (Ab130795). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.