abbit anti Human Thioredoxin/TRX Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on. Application: WB: 1/1000-1/10000 IHC: 1/50-1/200 IF/ICC: 1/100-1/500 Protein Function: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
Recombinant Thioredoxin/TRX Antibody (Ab130970) - Western Blot All lanes: Recombinant Thioredoxin/TRX Antibody (Ab130970) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 12 kDa Observed band size: 10,13 kDa Exposure time: 5.1 s
Recombinant Thioredoxin/TRX Antibody (Ab130970) - IF/ICC Immunocytochemistry analysis of Thioredoxin (green) in HeLa using Recombinant Thioredoxin/TRX Antibody (Ab130970), and cells were counterstained with DAPI (blue).
Recombinant Thioredoxin/TRX Antibody (Ab130970) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant Thioredoxin/TRX Antibody (Ab130970). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.