Rabbit anti Human Topoisomerase I Antibody, Recombinant, could be used for WB, IHC, ICC/IF and so on. Application: WB: 1/500 – 1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
规格
产品名称
Recombinant Topoisomerase I Antibody
别名
拓扑异构酶Ⅰ重组兔单克隆抗体 | 重组拓扑异构酶 I 抗体
英文别名
DNA topoisomerase 1 antibody | DNA topoisomerase I antibody | NUP98 fusion gene antibody | TOP 1 antibody | TOP I antibody | TOP1 antibody | TOP1_HUMAN antibody | TOPI antibody | Topoisomerase (DNA) I antibody | Topoisomerase 1 antibody | Topoisomerase1 a
规格或纯度
ExactAb™, Validated, 重组, 0.2 mg/mL
宿主种属
兔(Rabbit)
特异性
Topoisomerase I
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human Topoisomerase I (AA 2-15).
阳性对照
WB: HEK293, MCF7, Hep G2, Jurkat, K562, HeLa, C6 and NIH/3T3 cel lysates. IHC: Human colon cancer tissue. ICC/IF: HeLa cells.
Recombinant Topoisomerase I Antibody (Ab131976) - Western Blot All lanes: Recombinant Topoisomerase I Antibody (Ab131976) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 91 kDa Observed band size: 95 kDa Exposure time: 1.5 s
Recombinant Topoisomerase I Antibody (Ab131976) - IF/ICC Immunocytochemistry analysis of Topoisomerase I (green) in HeLa using Recombinant Topoisomerase I Antibody (Ab131976) and cells were counterstained with DAPI (blue).
Recombinant Topoisomerase I Antibody (Ab131976) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant Topoisomerase I Antibody (Ab131976). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.