Protein Function Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.
规格
产品名称
Recombinant TPX2 Antibody
别名
微管相关同源蛋白TPX2抗体 | 重组TPX2抗体
英文别名
C20ORF1 antibody | C20orf2 antibody | Chromosome 20 Open Reading Frame 1 antibody | Differentially expressed in cancerous and non-cancerous lung cells 2 antibody | Differentially expressed in cancerous and noncancerous lung cells 2 antibody | Differential
规格或纯度
ExactAb™, Validated, 重组, 0.5 mg/mL
宿主种属
兔(Rabbit)
特异性
TPX2
种属反应性
人(Human)
免疫原
A synthetic peptide derived from human TPX2 (AA 570-589)
阳性对照
WB: HeLa, MCF7 and HEK293 cell lysates. IHC: Human lung cancer tissue. ICC/IF: HeLa cells.
Recombinant TPX2 Antibody (Ab132114) - Western Blot All lanes: Recombinant TPX2 Antibody (Ab132114) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 50.5 s
Recombinant TPX2 Antibody (Ab132114) - IF/ICC Immunocytochemistry analysis of TPX2 (green) in HeLa using Recombinant TPX2 Antibody (Ab132114) and DAPI (blue).
Recombinant TPX2 Antibody (Ab132114) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant TPX2 Antibody (Ab132114). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.