Rabbit anti Human TRIM25 Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.
规格
产品名称
Recombinant TRIM25 Antibody
别名
重组TRIM25抗体
英文别名
2.3.2.27 | EC 6.3.2.n3 | Estrogen-responsive finger protein | RING finger protein 147 | RING-type E3 ubiquitin transferase | RING-type E3 ubiquitin transferase TRIM25 | Tripartite motif-containing protein 25 | Ubiquitin/ISG15-conjugating enzyme TRIM25 | Z
规格或纯度
ExactAb™, Validated, 重组, 0.5 mg/mL
宿主种属
兔(Rabbit)
特异性
TRIM25
种属反应性
人(Human),大鼠(Rat)
免疫原
A synthetic peptide derived from human TRIM25 (AA 142-157)
阳性对照
WB: K562, HeLa and C6 cell lysates. ICC/IF: MCF7 cells.
Recombinant TRIM25 Antibody (Ab132484) - Western Blot All lanes: Recombinant TRIM25 Antibody (Ab132484) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 71 kDa Observed band size: 69 kDa Exposure time: 17.0 s
Recombinant TRIM25 Antibody (Ab132484) - Western Blot All lanes: Recombinant TRIM25 Antibody (Ab132484) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 71 kDa Observed band size: 69 kDa Exposure time: 1.0 s
Recombinant TRIM25 Antibody (Ab132484) - IF/ICC Immunocytochemistry analysis of TRIM25/EFP (green) in MCF7 using Recombinant TRIM25 Antibody (Ab132484), and cells were counterstained with DAPI (blue).