Rabbit anti Human UBE2C Antibody, Polyclonal, could be used for WB, ICC, IF and so on. Application · WB: 0.01-2μg/mL · ICC/IF: 5-20μg/mL Protein Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
UBE2C Antibody (Ab133227) - Western Blot All lanes: UBE2C Antibody (Ab133227) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 20 kDa Observed band size: 17, 19 kDa Exposure time: 5.0 s
UBE2C Antibody (Ab133227) - IF/ICC FITC staining on IF Samples: Human HeLa Cells Primary Ab: 20 µg/mL UBE2C Antibody (Ab133227).
UBE2C Antibody (Ab133227) - IF/ICC
IF analysis of UBE2C Antibody (Ab133227) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with UBE2C Antibody (Ab133227) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
ZJ24F0404092
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