Rabbit anti Human UBE4B Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500 - 1/1000 ICC/IF: 1/50-1/200 Protein Function Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3. Post-translational Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7.
Recombinant UBE4B Antibody (Ab133294) - Western Blot All lanes: Recombinant UBE4B Antibody (Ab133294) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 146 kDa Observed band size: 138 kDa Exposure time: 5.4 s
Recombinant UBE4B Antibody (Ab133294) - IF/ICC Immunocytochemistry analysis of UBE4B (green) in MCF7 using Recombinant UBE4B Antibody (Ab133294) and DAPI (blue).