UBQLN2 Antibody (Ab133349) - Western Blot All lanes: UBQLN2 Antibody (Ab133349) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 7.8 s
UBQLN2 Antibody (Ab133349) - IF/ICC
IF analysis of UBQLN2 Antibody (Ab133349) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with UBQLN2 Antibody (Ab133349) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).