Rabbit anti Human UFM1 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500 - 1/1000 IHC: 1/50-1/100 Protein Function Ubiquitin-like modifier protein which binds to a number of target proteins, such as DDRGK1.
Recombinant UFM1 Antibody (Ab133422) - Western Blot All lanes: Recombinant UFM1 Antibody (Ab133422) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 9 kDa Observed band size: 12 kDa Exposure time: 26.4 s
Recombinant UFM1 Antibody (Ab133422) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant UFM1 Antibody (Ab133422). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant UFM1 Antibody (Ab133422) - IF/ICC IF analysis of Recombinant UFM1 Antibody (Ab133422) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant UFM1 Antibody (Ab133422) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab138205) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).