Rabbit anti Human UQCRB Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000 IHC: 1/50-1/100 IF/ICC: 1/200
Protein Function:
This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This component is involved in redox-linked proton pumping.
Recombinant UQCRB Antibody (Ab133582) - Western Blot All lanes: Recombinant UQCRB Antibody (Ab133582) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 20.0 s
Recombinant UQCRB Antibody (Ab133582) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant UQCRB Antibody (Ab133582). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant UQCRB Antibody (Ab133582) - IF/ICC IF analysis of Recombinant UQCRB Antibody (Ab133582) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant UQCRB Antibody (Ab133582) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).