Recombinant UQCRH Antibody (Ab133593) - Western Blot All lanes: Recombinant UQCRH Antibody (Ab133593) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/10000 dilution
Predicted band size: 11 kDa Observed band size: 13 kDa Exposure time: 100.0 s
Recombinant UQCRH Antibody (Ab133593) - Western Blot All lanes: Recombinant UQCRH Antibody (Ab133593) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/10000 dilution
Predicted band size: 11 kDa Observed band size: 13 kDa Exposure time: 100.0 s
Recombinant UQCRH Antibody (Ab133593) - IF/ICC Immunocytochemistry analysis of UQCRH (green) in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Recombinant UQCRH Antibody (Ab133593) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).