ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human USP30 Antibody, Polyclonal, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/1000
ICC/IF: 1/100-1/500
Protein Function：
May participate in the maintenance of mitochondrial morphology.
Recombinant USP30 Antibody (Ab133739) - Western Blot
All lanes: Recombinant USP30 Antibody (Ab133739) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/40000 dilution
Predicted band size: 59 kDa
Observed band size: 61 kDa
Exposure time: 3.9 s
Recombinant USP30 Antibody (Ab133739) - IF
Immunofluorescent analysis of USP30 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the Recombinant USP30 Antibody (Ab133739) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
1/500-1/1000
1/100-1/500
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1215436 |
分析证书 | Ab133739 |
| ZJ24F1215437 |
分析证书 | Ab133739 |
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