ExactAb™,已验证,无载体,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human VCP Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 0.1-2 µg/mL
IHC: 5-20 µg/mL
Protein Function:
Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Regulates E3 ubiquitin-protein ligase activity of RNF19A.
VCP Antibody (Ab133987) - Western Blot
All lanes: VCP Antibody (Ab133987) at 1/3000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 89 kDa
Observed band size: 93 kDa
Exposure time: 5.0 s
VCP Antibody (Ab133987) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 20 µg/mL VCP Antibody (Ab133987)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
VCP Antibody (Ab133987) - IHC
DAB staining on IHC
Samples: Human Stomach Tissue
Primary Ab: 20 µg/mL VCP Antibody (Ab133987)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
VCP Antibody (Ab133987) - IHC
DAB staining on IHC
Samples: Human Stomach cancer Tissue
Primary Ab: 20 µg/mL VCP Antibody (Ab133987)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
VCP Antibody (Ab133987) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 20 µg/mL VCP Antibody (Ab133987)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
VCP Antibody (Ab133987) - IF/ICC
IF analysis of VCP Antibody (Ab133987) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with VCP Antibody (Ab133987) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
5-20 µg/mL
0.1-2 µg/mL
2.5 µg/mL
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
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通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0505634 |
分析证书 | Ab133987 |
| ZJ24F0505635 |
分析证书 | Ab133987 |
| ZJ24F0505636 |
分析证书 | Ab133987 |
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