Rabbit anti Human VPS4a Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500 - 1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis.
规格
产品名称
Recombinant VPS4a Antibody
别名
液泡分选蛋白4抗体
英文别名
Protein SKD2 antibody | hVPS4 antibody | SKD1 antibody | SKD1 homolog antibody | SKD1A antibody | SKD2 antibody | Vacuolar protein sorting 4 homolog A antibody | Vacuolar protein sorting 4, yeast, homolog of, A antibody | Vacuolar protein sorting factor 4
规格或纯度
ExactAb™, Validated, 重组
宿主种属
兔(Rabbit)
特异性
VPS4A
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human VPS4a (AA 424-437).
阳性对照
WB: K562, HeLa, Jurkat, Hep G2, RAW 264.7, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer tissue. IF/ICC: HeLa cells.
Recombinant VPS4a Antibody (Ab134482) - Western Blot All lanes: Recombinant VPS4a Antibody (Ab134482) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 49 kDa Observed band size: 52 kDa Exposure time: 29.1 s
Recombinant VPS4a Antibody (Ab134482) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant VPS4a Antibody (Ab134482). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant VPS4a Antibody (Ab134482) - IF/ICC Immunocytochemistry analysis of VPS4a(green) in HeLa using Recombinant VPS4a Antibody (Ab134482), and DAPI(blue).