ExactAb™,已验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human Wnt5a Antibody, Recombinant, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Protein Function
Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.
Recombinant Wnt5a Antibody (Ab134765) - Western Blot
All lanes: Recombinant Wnt5a Antibody (Ab134765) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 45 kDa
Exposure time: 6.3 s
Recombinant Wnt5a Antibody (Ab134765) - Western Blot
All lanes: Recombinant Wnt5a Antibody (Ab134765) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 45 kDa
Exposure time: 1.0 s
Recombinant Wnt5a Antibody (Ab134765) - IF/ICC
Immunocytochemistry analysis of Wnt5a (green) in HeLa using Recombinant Wnt5a Antibody (Ab134765), and cells were counterstained with DAPI (blue).
1/500-1/1000
1/50-1/200
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1011838 |
分析证书 | Ab134765 |
| ZJ23F1001323 |
分析证书 | Ab134765 |
| ZJ23F1001324 |
分析证书 | Ab134765 |
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