ExactAb™,已验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human WTAP Antibody, Recombinant, could be used for WB, IHC and so on.
Application
· WB: 1/500-1/1000
· IHC: 1/50-1/100
Protein Function
Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation.
Recombinant WTAP Antibody (Ab134816) - Western Blot
All lanes: Recombinant WTAP Antibody (Ab134816) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 54 kDa
Exposure time: 48.8 s
Recombinant WTAP Antibody (Ab134816) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant WTAP Antibody (Ab134816). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant WTAP Antibody (Ab134816) - IF/ICC
IF analysis of Recombinant WTAP Antibody (Ab134816) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant WTAP Antibody (Ab134816) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
1/500-1/1000
1/50 - 1/100
1:500
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0505950 |
分析证书 | Ab134816 |
| ZJ24F0505951 |
分析证书 | Ab134816 |
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