Rabbit anti Human WWOX Antibody, Recombinant, could be used for WB, IHC and so on. Application
WB: 1/500-1/1000 IHC: 1/50-1/100
IF/ICC: 1/200 Protein Function Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.
规格
产品名称
Recombinant WWOX Antibody
别名
包含氧化还原酶的WW域抗体 | 重组WWOX抗体
英文别名
EC 1.1.1.- | Fragile site FRA16D oxidoreductase | Short chain dehydrogenase/reductase family 41C member 1 | 5330426P09Rik antibody | 9030416C10Rik antibody | Aberrant WW domain-containing oxidoreductase antibody | D16S432E antibody | EC 1.1.1.- antibody |
规格或纯度
ExactAb™, Validated, 重组, 0.5 mg/mL
宿主种属
兔(Rabbit)
特异性
WWOX
种属反应性
人(Human),小鼠(Mouse)
免疫原
Recombinant full length protein (Mouse)
阳性对照
WB: Mouse testis, Mouse spleen, U-2 OS, HeLa, Hep G2, MCF7 and HEK293 lysates. IHC: Human lung cancer tissue. IF/ICC: HeLa cells.
Recombinant WWOX Antibody (Ab134823) - Western Blot All lanes: Recombinant WWOX Antibody (Ab134823) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 47 kDa Observed band size: 43 kDa Exposure time: 4.5 s
Recombinant WWOX Antibody (Ab134823) - Western Blot All lanes: Recombinant WWOX Antibody (Ab134823) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 47 kDa Observed band size: 43 kDa Exposure time: 15.0 s
Recombinant WWOX Antibody (Ab134823) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant WWOX Antibody (Ab134823). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant WWOX Antibody (Ab134823) - IF/ICC IF analysis of Recombinant WWOX Antibody (Ab134823) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant WWOX Antibody (Ab134823) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).