计算溶液所需的质量、体积或浓度。
Recombinant CD20 Antibody (PerCP) (Ab169187)-Flow
Raji Cells are stained with Recombinant CD20 Antibody (PerCP) (Ab169187) (Red). Unstained Raji Cells (Gray) are used as control.
Em:675nm,Ex:482nm,ExactAb™,已验证,无叠氮钠,重组 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光,禁止冷冻。低温运输,禁止冷冻 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
背景:
CD20是一种分子量在33-37kDa的非糖基化磷蛋白,其结构中包含四个非连续跨膜结构域,属于白细胞分化抗原家族,通常表达于除浆细胞(分泌免疫球蛋白的B细胞)外的发育分化各阶段的B细胞的表面,通过调节跨膜钙离子流动直接对B细胞起作用,在B细胞增殖和分化中起重要的调节作用。
推荐使用浓度:
流式:10uL /Test
Background:
The recombinant monoclonal antibody specifically binds to CD20, encoded by the MS4A1 (Membrane-spanning 4-domains, subfamily A, member 1) gene. CD20 is a 33-37kDa, unglycosylated four-transmembrane phosphoprotein. CD20 is expressed on pre-B-cells, resting and activated B cells, and follicular dendritic cells, but not plasma cells. Low level CD20 expression is observed on a small subset of normal circulating T lymphocytes. The CD20 molecule is involved in the regulation of B-cell activation and proliferation.
PerCP conjugates are excited by the 488 nm line of the argon-ion laser and have a large Stokes shift, providing minimal spectral overlap into the R-PE (phycoerythrin) channel.
Conjugation:
Purified antibody is conjugated with PerCP under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Recommended Usage:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 10 µL of antibody per test (million cells in 100 µL staining volume ). Since applications vary, the appropriate dilutions must be determined for individual use.
Recombinant CD20 Antibody (PerCP) (Ab169187)-Flow
Raji Cells are stained with Recombinant CD20 Antibody (PerCP) (Ab169187) (Red). Unstained Raji Cells (Gray) are used as control.
10µL/Test
For flow cytometric staining, the suggested use of this reagent is 10 µL per million cells in 100 µL staining volume or 10 µL per 100 µL of whole blood.
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab169187 | |
| 分析证书 | Ab169187 |
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查看 Recombinant 级别指南 → 查看 Validated 级别指南 → 查看 ExactAb™ 级别指南 → 查看 Azide Free 级别指南 → 查看 Ex:482nm 级别指南 → 查看 Em:675nm 级别指南 →