背景:
CD20是一种分子量在33-37kDa的非糖基化磷蛋白,其结构中包含四个非连续跨膜结构域,属于白细胞分化抗原家族,通常表达于除浆细胞(分泌免疫球蛋白的B细胞)外的发育分化各阶段的B细胞的表面,通过调节跨膜钙离子流动直接对B细胞起作用,在B细胞增殖和分化中起重要的调节作用。
推荐使用浓度:
流式:1-5µg/mL
Background:
The recombinant monoclonal antibody specifically binds to CD20, encoded by the MS4A1 (Membrane-spanning 4-domains, subfamily A, member 1) gene. CD20 is a 33-37kDa, unglycosylated four-transmembrane phosphoprotein. CD20 is expressed on pre-B-cells, resting and activated B cells, and follicular dendritic cells, but not plasma cells. Low level CD20 expression is observed on a small subset of normal circulating T lymphocytes. The CD20 molecule is involved in the regulation of B-cell activation and proliferation.
Conjugation:
The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Recommended Usage:
Each lot of this antibody is quality control tested by flow cytometric analysis. The reagent is suggested to be used at 1-5µg/mL per test (million cells in 100 µL staining volume ). Since applications vary, the appropriate dilutions must be determined for individual use.