PBS Only,已验证,无叠氮钠,无载体,高性能 级 提供 ≥95%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Application
WB: 1/1,000 - 1/200,000; Predicted molecular weight: 61.0 KDa
ICC/IF: 1/200
Flow: 1/800 - 1/200,000
Hsp60 Mouse mAb (Ab169190) - Western Blot
All lanes: Hsp60 Mouse Antibody (Ab169190) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 58 kDa
Exposure time: 15.0 s
Hsp60 Mouse mAb (Ab169190) - Western Blot
All lanes: Hsp60 Mouse Antibody (Ab169190) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 4.0 s
Hsp60 Mouse mAb (Ab169190) - Flow Cytometry
Overlay histogram showing Jurkat cells stained with Hsp60 Mouse mAb (Ab169190) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).
Hsp60 Mouse mAb (Ab169190) - ICC/IF
IF analysis of Hsp60 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Hsp60 Mouse mAb (Ab169190) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
1/200
1/800 - 1/200,000
1/1,000 - 1/200,000; Predicted molecular weight: 61.0 KDa
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1114042 |
分析证书 | Ab169190 |
| ZJ24F1114043 |
分析证书 | Ab169190 |
| ZJ24F1114044 |
分析证书 | Ab169190 |
| ZJ24F0809905 |
分析证书 | Ab169190 |
| ZJ24F0809906 |
分析证书 | Ab169190 |
| ZJ24F0809932 |
分析证书 | Ab169190 |
| ZJ24F0606530 |
分析证书 | Ab169190 |
| ZJ24F0606531 |
分析证书 | Ab169190 |
| ZJ24F0606532 |
分析证书 | Ab169190 |
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查看 Carrier Free 级别指南 → 查看 Validated 级别指南 → 查看 Azide Free 级别指南 → 查看 High Performance 级别指南 → 查看 PBS Only 级别指南 →
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