ExactAb™,PBS Only,已验证,无叠氮钠,无载体,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Application:
WB: 1/1,000-1/200,000 ; Predicted molecular weight: 124 kDa
Flow: 0.04 μg/mL - 2.5 μg/mL
ICC/IF: 5.0 μg/mL
Vinculin Mouse mAb (Ab169192) - Western Blot
All lanes: Vinculin Antibody (Ab169192) at 0.5 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 124.0 kDa
Observed band size: 125.8 kDa
Exposure time: 6.879 s
Vinculin Mouse mAb (Ab169192) - Western Blot
All lanes: Vinculin Antibody (Ab169192) at 0.5 μg/mL
Samples: Lysate at 20 µg
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 124.0 kDa
Observed band size: 122.0 kDa
Exposure time: 40.1 s
Vinculin Mouse mAb (Ab169192) - Western Blot
All lanes: Vinculin Antibody (Ab169192) at 0.5 μg/mL
Samples: Lysate at 20 µg
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 124.0 kDa
Observed band size: 122.5 kDa
Exposure time: 0.1 s
Vinculin Mouse mAb (Ab169192) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Vinculin Mouse mAb (Ab169192) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).
Vinculin Mouse mAb (Ab169192) - ICC/IF
IF analysis of Vinculin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Vinculin Mouse mAb (Ab169192) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
0.04 μg/mL - 2.5 μg/mL
5.0 μg/mL
1/1,000-1/200,000 ; Predicted molecular weight: 124 kDa
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1114030 |
分析证书 | Ab169192 |
| ZJ24F1114029 |
分析证书 | Ab169192 |
| ZJ24F1114028 |
分析证书 | Ab169192 |
| ZJ24F0102566 |
分析证书 | Ab169192 |
| ZJ24F0102560 |
分析证书 | Ab169192 |
| ZJ24F0102559 |
分析证书 | Ab169192 |
| ZJ24F0102558 |
分析证书 | Ab169192 |
| ZJ23F1101843 |
分析证书 | Ab169192 |
| ZJ23F1101844 |
分析证书 | Ab169192 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Carrier Free 级别指南 → 查看 Validated 级别指南 → 查看 ExactAb™ 级别指南 → 查看 Azide Free 级别指南 → 查看 High Performance 级别指南 → 查看 PBS Only 级别指南 →
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