Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Post-translational: Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques.Aceylated; mainly by myristic acid but also small amount of palmitic acid.
宿主种属
兔(Rabbit)
特异性
Vinculin
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human Vinculin (AA 1000-1078).
阳性对照
WB: MCF7, HeLa and C6 cell lysates. IF/ICC: A673 cells.
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant Vinculin Antibody (Ab134233) - Western Blot All lanes: Recombinant Vinculin Antibody (Ab134233) at 1/3000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 124 kDa Observed band size: 120 kDa Exposure time: 58.8 s
Recombinant Vinculin Antibody (Ab134233) - IF/ICC Immunofluorescent analysis of A673 cells, using Recombinant Vinculin Antibody (Ab134233).
ZJ25F0319812
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